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Thus, induction of HSP70 by heat stress enhances lipid accumulation in subcutaneous preadipocytes (Zhang et al., 2024)

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Complementary DNA (cDNA) was made by reverse transcription of RNA (2 μg) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2′-deoxy-uridine-5′-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)